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Immobilized metal affinity chromatography (IMAC) is an important tool for the purification of recombinant proteins with a cluster of histidine residues at the N- or C-terminus (also known as His-tagged proteins), or proteins naturally rich in histidine or cystine residues. Using IMAC, these proteins can be effectively captured from raw cell culture harvest followed by elution with an appropriate concentration of imidazole, and thus separated from impurity proteins and nucleic acids produced by the expression host cells. NanoMicro Technologies offers a wide variety of IMAC resins, pre-charged with Ni2+ or uncharged, for the preparation of highly pure recombinant proteins for scientific research, diagnostic assays and therapeutic applications.


  • Polymeric or cross-linked agarose particles with outstanding pressure-flow properties

  • Efficient mass transfer and high binding capacity; small elution volumes

  • Minimal non-specific binding; excellent His-tagged protein recovery

  • Low metal leaching level and excellent chemical compatibility

  • Uncharged resins available for the immobilization of divalent metals, such as Zn2+, Cu2+, Co2+, and Fe3+, for alternative protein binding selectivity

Product Name

NW Rose Ni FFNW Rose TED FFNW Rose IMAC FF
Chromatography TechniqueHistidine-tagged protein purification Histidine-tagged protein purification Histidine-tagged protein purification
MatrixCross-linked agarose with fast flow rateCross-linked agarose with fast flow rateCross-linked agarose with fast flow rate
Particle Size45-165 μm45-165 μm45-165 μm
LigandNTATEDNTA
Dynamic Binding Capacity~40 mg·mL-1~10 mg·mL-1~40 mg·mL-1
Metal Ion Capacity15 μmol·mL-1(Ni2+)45 μmol·mL-1(Ni2+)~15 μmol·mL-1(Ni2+)
Maximum Pressure0.3 MPa0.3 MPa0.3 MPa
Clean In Place0.5 M NaOH (Ni2+must be removed)0.5 M NaOH (Ni2+must be removed)0.5 M NaOH (Ni2+must be removed)
Maximum Flow Velocity150 cm/h50/30 Column, bed height 10 cm150 cm/h50/30 Column, bed height 10 cm150 cm/h50/30 Column, bed height 10 cm
Recommended Flow Velocity600 cm/h, 16/20 Column, bed height 10 cm600 cm/h, 16/20 Column, bed height 10 cm600 cm/h, 16/20 Column, bed height 10 cm
pH Stability

3-12 (operational),

2-14 (CIP, Ni2+must be removed)

3-12 (operational), 2-14 (CIP)

3-12 (operational),

2-14 (CIP, Ni2+must be removed)

Chemical StabilityStable in commonly used aqueous buffers, 0.1 M HCl, 0.1 M NaOH, 2% SDS, 30% isopropyl alcohol, 6 M guanidine hydrochloride, 8 M urea, 70% ethanol (if Ni2+ is removed)Stable in commonly used aqueous buffers, 0.1 M HCl, 0.1 M NaOH, 2% SDS, 30% isopropyl alcohol, 6 M guanidine hydrochloride, 8 M urea, 70% ethanol (if Ni2+ is removed)Stable in commonly used aqueous buffers, 0.1 M HCl, 0.1 M NaOH, 2% SDS, 30% isopropyl alcohol, 6 M guanidine hydrochloride, 8 M urea, 70% ethanol (if Ni2+ is removed)
Operational Temperature4-304-304-30
Storage20% ethanol, 4-30 20% ethanol, 4-30 20% ethanol, 4-30


Product Name

Pack Size

Cat. No.

NW Rose Ni FF 5mL

5mL

60042-531800-2005

NW Rose Ni FF 25mL

25mL

60042-531800-2025

NW Rose Ni FF 100mL

100mL

60042-531800-2100

NW Rose Ni FF 200mL

200mL

60042-531800-2200

NW Rose Ni FF 200mL

200mL

60042-531800-2200

NW Rose Ni FF 500mL

500mL

60042-531800-2500

NW Rose Ni FF 1L

1L

60042-531800-1001

NW Rose Ni FF 5L

5L

60042-531800-1005

NW Rose Ni FF 10L

10L

60042-531800-1010

NW Rose Ni FF 20L

20L

60042-531800-1020

NW Rose TED FF 5mL

5mL

60042-371301-2005

NW Rose TED FF 25mL

25mL

60042-371301-2025

NW Rose TED FF 100mL

100mL

60042-371301-2100

NW Rose TED FF 200mL

200mL

60042-371301-2200

NW Rose TED FF 200mL

200mL

60042-371301-2200

NW Rose TED FF 500mL

500mL

60042-371301-2500

NW Rose TED FF 1L

1L

60042-371301-1001

NW Rose TED FF 5L

5L

60042-371301-1005

NW Rose TED FF 10L

10L

60042-371301-1010

NW Rose TED FF 20L

20L

60042-371301-1020

NW Rose IMAC FF 5mL

5mL

60042-092100-2005

NW Rose IMAC FF 25mL

25mL

60042-092100-2025

NW Rose IMAC FF 100mL

100mL

60042-092100-2100

NW Rose IMAC FF 200mL

200mL

60042-092100-2200

NW Rose IMAC FF 200mL

200mL

60042-092100-2200

NW Rose IMAC FF 500mL

500mL

60042-092100-2500

NW Rose IMAC FF 1L

1L

60042-092100-1001

NW Rose IMAC FF 5L

5L

60042-092100-1005

NW Rose IMAC FF 10L

10L

60042-092100-1010

NW Rose IMAC FF 20L

20L

60042-092100-1020