NMab ProtG Protein G Affinity Chromatography Resin
NMab ProtG is an affinity resin prepared by immobilizing Protein G onto an agarose matrix via epoxy activation. Protein G exhibits a broader binding spectrum, with strong binding to the Fc fragment of antibodies as well as weak interactions with the Fab fragment. Therefore, NMab ProtG is commonly used for the purification of antibodies or antibody fragments from cell culture harvests, as well as other antibodies from various species sera.
Performance Specifications
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| Product Model | NMab ProtG |
| Separation Principle | Protein G affinity capture |
| Matrix | Highly cross-linked agarose |
| Ligand Coupling Method | Epoxy coupling |
| Dynamic Binding Capacity | 55-65 mg·mL-1 (human IgG, 5 min residence time) |
| Maximum Operating Pressure | 0.3 MPa |
| CIP (Clean-in-Place) | 6 M guanidine hydrochloride |
| Recommended Flow Rate | 100-500 cm/h |
| pH Stability | 2-10 |
| Chemical Stability | Compatible with all commonly used buffers, 20 mM sodium phosphate, 1% SDS, 6 M guanidine hydrochloride, 70% ethanol, 6 M urea, and other common organic solvents; avoid prolonged exposure to strong acids and bases. |
| Operating Temperature | 2-40 ℃ |
| Storage | 2-8 ℃, 20% ethanol |
Ordering Information
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| Product | Package Size | Catalog # |
| NMab ProtG | 2 mL | 17015-090100-2002 |
| 5 mL | 17015-090100-2005 | |
| 25 mL | 17015-090100-2025 | |
| 100 mL | 17015-090100-2100 | |
| 1 L | 17015-090100-1001 |
Note: Pre-packed columns are available in 7.7 mm × 22 mm, 16 mm × 25 mm, and 7.7 mm × 100 mm dimensions. For additional sizes or custom requirements, please contact us.