NW Rose Plus Plasmid Plasmid Affinity Chromatography Resin

NW Rose Plus Plasmid is an affinity chromatography resin designed for the purification of supercoiled plasmid DNA. It is prepared by immobilizing the sulfur-containing compound 2-mercaptopyridine onto high-flow agarose microspheres. The optimized ligand density provides appropriate affinity for supercoiled DNA, while the high-flow agarose matrix enhances the binding capacity for large-molecular-weight supercoiled plasmid DNA. Thiophilic affinity chromatography separates and purifies supercoiled plasmids based on the interaction between electron donors and electron acceptors, which is enhanced under high-salt conditions and weakened under low-salt conditions.