UniHR Phenyl-80L HIC Resin
Hydrophobic interaction chromatography (HIC) separates biomolecules based on differences in their surface hydrophobicity. Under high-salt conditions, biomolecules adsorb onto the hydrophobic ligands of the HIC resin. By decreasing the salt concentration, proteins are eluted in order of increasing hydrophobicity, achieving separation and purification. HIC offers mild operating conditions and high resolution, making it a widely used method for biomolecule purification.
NanoMicro Uni®HR HIC resins are based on monodisperse, highly cross-linked PMMA (polymethyl methacrylate) hydrophilic-modified microspheres. Phenyl and butyl hydrophobic groups are immobilized on the surface via advanced bonding chemistry. These resins adsorb biomolecules under high-salt conditions and elute under low-salt conditions. They offer high binding capacity and low non-specific adsorption, making them ideal for further purification following ion-exchange chromatography.
Product Advantages:
a) Monodisperse microspheres for superior batch-to-batch consistency
b) Patented surface bonding chemistry for higher dynamic binding capacity
c) Significantly lower compression factor than agarose and dextran gels
d) Comprehensive resin portfolio with customized solutions available
e) High mechanical strength and lower back pressure for improved productivity
Performance Specifications
| Name | UniHR Phenyl-80L |
| Separation Principle | Hydrophobic interaction |
| Matrix | Polymethacrylate (PMMA) |
| Particle Size | 80 µm |
| Ligand | Phenyl |
| Dynamic Binding Capacity | ~10 mg·mL-1 (Lys) |
| Maximum Pressure | 0.5 MPa |
| CIP (Clean-in-Place) | 1 M NaOH / 70% Ethanol |
| Recommended Flow Rate | 150-750 cm/h |
| pH Stability | 2-12 |
| Chemical Stability | All commonly used buffers, 1 M acetic acid, 1 M NaOH, 1 M HCl, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 6 M guanidine-HCl, 8 M urea and other common organic solvents; avoid contact with strong oxidizing agents. |
| Operating Temperature | 15-30 ℃ |
| Storage Conditions | 20% Ethanol, 4-25 ℃ |
Ordering Information
| Product | Package Size | Catalog # |
| Uni®HR Phenyl-80L | 30 mL | 06131-080100-2030 |
| 50 mL | 06131-080100-2050 | |
| 100 mL | 06131-080100-2100 | |
| 300 mL | 06131-080100-2300 | |
| 500 mL | 06131-080100-2500 | |
| 1 L | 06131-080100-1001 | |
| 5 L | 06131-080100-1005 | |
| 10 L | 06131-080100-1010 | |
| 50 L | 06131-080100-1050 | |
| 100 L | 06131-080100-1100 |