UniGel-65SP HC Ion Exchange Chromatography Resin
Ion Exchange Chromatography (IEX) separates biomolecules based on differences in their surface charge (type, number, and distribution). NanoMicro offers a comprehensive range of IEX resins based on both monodisperse and polydisperse polymer microspheres. These resins feature hydrophilic surface modification followed by bonding of ion exchange groups, and are available in multiple series to meet the demands of capture, intermediate purification, polishing, and analytical purification. With excellent biomolecule compatibility and bed stability, our solutions support bioprocessing from laboratory to industrial scale.
Key advantages of UniGel-65SP HC:
1) Macroporous PMMA matrix with high rigidity, ideal for scale-up;
2) Optimized ligand with enhanced salt tolerance;
3) Unique surface hydrophilization process minimizes non-specific binding;
1) Low compression and swelling factors (significantly lower than agarose and dextran gels) ensure high bed stability;
2) High-strength matrix enables faster flow rates and taller beds, improving purification efficiency;
3) Superior alkali resistance extends resin lifetime, increases productivity, reduces production costs, and enhances operational efficiency.
Performance Specifications
| Product Model | UniGel-65SP HC |
| Separation Principle | Strong cation exchange |
| Matrix | Polymethacrylate (PMMA) |
| Particle Size | ~ 65 μm |
| Ligand | —(CH2)3SO3- |
| Dynamic Binding Capacity | ~115 mg/mL (IgG) |
| Ionic Capacity | 0.13 meq·mL-1 |
| Max Operating Pressure | 0.5 MPa |
| CIP | 0.5 M NaOH |
| Recommended Flow Rate | 150-750 cm/h |
| pH Stability | 2~12 |
| Chemical Stability | Compatible with all common buffers, 1 M acetic acid, 1 M NaOH, 1 M HCl, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 6 M guanidine HCl, 8 M urea, and other common organic solvents; avoid strong oxidizing agents. |
Ordering Information
| Product | Package Size | Catalog # |
| UniGel-65SP HC | 30 mL | 04082-165100-2030 |
| 50 mL | 04082-165100-2050 | |
| 100 mL | 04082-165100-2100 | |
| 300 mL | 04082-165100-2300 | |
| 500 mL | 04082-165100-2500 | |
| 1 L | 04082-165100-1001 | |
| 5 L | 04082-165100-1005 | |
| 10 L | 04082-165100-1010 | |
| 50 L | 04082-165100-1050 | |
| 100 L | 04082-165100-1100 |