HAPcera Type II Hydroxyapatite Chromatography Media

Ceramic hydroxyapatite is a hydroxy compound multimodal chromatography media with significant application value. It features a spherical morphology and an ultra-large pore size. Unlike traditional chromatography media, hydroxyapatite possesses both metal affinity and ion exchange capabilities, offering unique separation performance and providing distinctive and effective purification solutions. Hydroxyapatite chromatography media can be used to purify a wide range of biomolecules, including:

Different types of monoclonal and polyclonal antibodies
Bispecific antibodies and antibody fragments
Viral particles
Vaccines
Recombinant proteins
Enzymes
Supercoiled DNA, single-stranded or double-stranded DNA

NanoMicro offers three types of hydroxyapatite chromatography media: HAPcera Type I, HAPcera Type II, and HAPcera-D.

HAPcera Type I features a smaller pore size, suitable for the purification of small proteins and monoclonal antibodies;

HAPcera-D features a larger pore size, suitable for the purification of large molecules such as bispecific antibodies and HPV vaccines;

HAPcera Type II features an ultra-large pore size, suitable for the purification of very large biomolecules such as viruses, Ig M, VLP particles, and plasmids.

Performance Specifications

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Product Model	HAPcera Type I	HAPcera-D	HAPcera Type II
Functional Group	Ca²⁺，PO₄^3-，-OH
Particle Size	40±4 μm
Packed Density	0.72 g/mL
Binding Capacity (IgG)*	~35 mg/mL	~20 mg/mL	~15 mg /mL
Recommended Flow Rate	50-1000 cm/h
Sanitization	1-2 M NaOH
pH Stability	6.5-14, storable in 1 M NaOH for over 1 year
Regeneration	0.4 M sodium phosphate buffer, pH 7-7.5;
	1 M trisodium phosphate buffer, pH 11-12;
	For higher phosphate concentrations, 0.4-1 M potassium phosphate buffer can be used
Autoclaving	121 ℃, 20 min, phosphate buffer, pH 7
Storage Conditions**	0.1 M NaOH, room temperature

*Dynamic binding capacity (10% breakthrough): tested on a 7.7 mm I.D. × 22 mm H column, 1 min residence time, loading 1 mg/mL IgG (10 mM sodium phosphate, pH 7.0)

**Store as a dry powder in a sealed, dry container at room temperature (2-30℃) before opening. The conditions listed here apply after opening.

Ordering Information

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Product	Package Size	Catalog #
HAPcera Type I	10 g	80000-040001-4010
	100 g	80000-040001-4100
	1 KG	80000-040001-3001
	5 KG	80000-040001-3005
HAPcera Type II	10 g	80000-040002-4010
	100 g	80000-040002-4100
	1 KG	80000-040002-3001
	5 KG	80000-040002-3005
HAPcera-D	10 g	80000-040003-4010
	100 g	80000-040003-4100
	1 KG	80000-040003-3001
	5 KG	80000-040003-3005

Note: Pre-packed columns are available in 7.7 mm × 22 mm, 16 mm × 25 mm, and 7.7 mm × 100 mm sizes. For additional specifications or custom requirements, please contact us.