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NW Super 75 Size Exclusion Chromatography Resin

NW Super 75 is an agarose-based size exclusion chromatography resin designed for the separation and purification of biomolecules. Manufactured from agarose and highly cross-linked dextran, these hydrophilic, porous microspheres are ideal for high-resolution purification of proteins, polysaccharides, nucleic acids, and other biological samples. It is the preferred resin for biomolecules (proteins).

Key features of NW Super 75 size exclusion media:

  • Uniform particle size for high resolution, typically achieving column efficiency above 10,000 plates/m

  • Separation achievable at high flow rates

  • High chemical and physical stability

  • High recovery yields

  • Easy scale-up

Performance Specifications

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Product ModelNW Super 75
Separation PrincipleSize exclusion
MatrixHighly cross-linked agarose and dextran microspheres
Average Particle Size (swollen)24-44 μm
Separation Range (globular proteins)3~70 KD
Maximum Flow Rate90 cm/h
Recommended Flow Rate Range30-60 cm/h
Maximum Pressure0.5 MPa
pH Stability3-12 (working), 1-14 (CIP)
Chemical StabilityCommon aqueous solutions, 1 M NaOH, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol
Operating Temperature4-40 ℃
Storage Conditions20% ethanol, 4-30 ℃


Ordering Information

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Product Package SizeCatalog #
NW Super 75150 mL60013-025000-2150
500 mL60013-025000-2500
1 L60013-025000-1001
5 L60013-025000-1005
10 L60013-025000-1010


Note: Pre-packed columns are available in 7.7 mm × 22 mm, 16 mm × 25 mm, and 7.7 mm × 100 mm dimensions. For additional specifications or custom requirements, please contact us.