NMOligo Series Solid Phase Synthesis for Oligonucleotides

Product Description

Introduction to Nucleic Acid Solid-phase Synthesis Supports

Nucleic acid solid-phase synthesis supports are critical materials in the chemical synthesis of oligonucleotides (oligonucleotide drugs). They are used to immobilize the initial nucleotide, providing a stable foundation for subsequent complex coupling reactions, ensuring that each nucleotide is accurately connected to progressively build oligonucleotides with specific sequences and functions. Currently, the phosphoramidite solid-phase synthesis method is predominantly used for oligonucleotide synthesis. Each complete synthesis cycle involves four steps and adds one nucleotide residue to the growing oligonucleotide chain attached to the solid support.

Figure 1. Solid-phase oligonucleotide synthesis steps

Currently, there are two mainstream support matrices on the market:

One is controlled pore glass (CPG) with inorganic matrix. In research settings, CPG is widely used, especially showing advantages in synthesizing longer nucleotide chains. However, CPG has loading capacity limitations, typically only 50-150 μmol/g, and is fragile, which poses constraints in scale-up synthesis and makes it difficult to meet large-scale production demands.

The other is polystyrene microspheres (PS-DVB) with organic matrix. The key feature of PS-DVB is its high loading capacity, generally reaching 350 μmol/g or above. Additionally, as an organic matrix, it offers good toughness, is not easily broken, and is widely used in large-scale synthesis.

NMOligo Series Solid-phase Synthesis supports for Oligonucleotides

NMOligo is a high-performance solid-phase synthesis support developed by NanoMicro based on years of PS-DVB polymer synthesis technology and surface modification methods. Utilizing NanoMicro's proprietary pore-forming technology, this support features uniform pore size and particle size distribution as well as an optimized cross-linked polystyrene structure, enabling high-purity, high-yield, and high-loading oligonucleotide synthesis while reducing solid-phase synthesis costs.

Figure 2. SEM image of NMOligo solid-phase synthesis support

The NMOligo series solid-phase synthesis supports offer the following advantages:

• High loading capacity: Advanced pore-forming and surface modification technologies enable high loading capacity, delivering excellent oligonucleotide synthesis yields;

• Superior batch-to-batch consistency: NMOligo manufacturing and loading comply with ISO 9001 quality management system standards, ensuring superior batch-to-batch consistency and providing reliable performance for oligonucleotide synthesis;

• Diverse custom product offerings: NMOligo solid-phase supports are available with various loading levels of standard ribose and deoxyribose bases, Unylinker, and various modified custom linkages;

• Reduced oligonucleotide synthesis costs: The high loading capacity, stable superior performance, and reasonable pricing of NMOligo reduce the synthesis cost per mole of oligonucleotide.