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Nucleic Acid Affinity Pre-packed Columns

NW Rose® Plasmid/NW Rose® Plus Plasmid are two thiophilic affinity media based on agarose microspheres, utilizing the interaction between electron donors and acceptors to purify supercoiled plasmids. Under high-salt conditions, the ligand binds to plasmids with high affinity; after impurities are removed, the plasmid can be eluted by lowering the salt concentration of the buffer solution. NanoGel™ dT20 features a PS-DVB rigid matrix with dT20 ligands covalently bonded via a linker on the surface, enabling affinity binding to the poly(A) tail at the 3' end of in vitro transcribed mRNA, greatly simplifying the mRNA capture process.

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Product Description

NW Rose® Plasmid/NW Rose® Plus Plasmid feature optimized 2-mercaptopyridine ligand density, maintaining high binding capacity for high-molecular-weight supercoiled DNA even at high flow rates.

NanoGel™ dT20 employs a monodisperse rigid hydrophilic PS-DVB matrix, offering alkali-resistant CIP, low ligand leaching, low non-specific adsorption, and support for high-throughput industrial production. The plasmid format efficiently enriches supercoiled plasmids and removes plasmid templates in a single step.

Fully system-compatible: compatible with AKTA and domestic bioprocess chromatography systems, with universal connectors and buffer systems fully compatible with imported media.

Performance Specifications

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Ordering Information

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Prepacked column specificationsCat. No.Prepacked column specificationsCat. No.
NmTRAP 1mL, NanoGel dT2077221-17030-050150NmTRAP 1mL, NW Rose Plasmid77221-60043-402400
NmTRAP 5mL, NanoGel dT2016255-17030-050150NmTRAP 5mL, NW Rose Plasmid16255-60043-402400
NmSCREEN 4.7mL, NanoGel dT2077105-17030-050150NmSCREEN 4.7mL, NW Rose Plasmid77105-60043-402400
NmPREP 16/100, NanoGel dT2016103-17030-050150NmPREP 16/100, NW Rose Plasmid16103-60043-402400
NmPREP 26/100, NanoGel dT2026153-17030-050150NmPREP 26/100, NW Rose Plasmid26153-60043-402400
NmVALID 8/100, NanoGel dT2008101-17030-050150NmVALID 8/100, NW Rose Plasmid08101-60043-402400
NmVALID 8/200, NanoGel dT2008201-17030-050150NmVALID 8/200, NW Rose Plasmid08201-60043-402400
NmVALID 11/100, NanoGel dT2011101-17030-050150NmVALID 11/100, NW Rose Plasmid11101-60043-402400
NmVALID 11/200, NanoGel dT2011201-17030-050150NmVALID 11/200, NW Rose Plasmid11201-60043-402400
NmVALID 16/100, NanoGel dT2016106-17030-050150NmVALID 16/100, NW Rose Plasmid16106-60043-402400
NmVALID 16/200, NanoGel dT2016206-17030-050150NmVALID 16/200, NW Rose Plasmid16206-60043-402400
NmVALID 26/100, NanoGel dT2026106-17030-050150NmVALID 26/100, NW Rose Plasmid26106-60043-402400
NmVALID 26/200, NanoGel dT2026206-17030-050150NmVALID 26/200, NW Rose Plasmid26206-60043-402400
NmVALID 35/100, NanoGel dT2035101-17030-050150NmVALID 35/100, NW Rose Plasmid35101-60043-402400
NmVALID 35/200, NanoGel dT2035201-17030-050150NmVALID 35/200, NW Rose Plasmid35201-60043-402400
NmVALID 50/100, NanoGel dT2050106-17030-050150NmVALID 50/100, NW Rose Plasmid50106-60043-402400
NmVALID 50/200, NanoGel dT2050206-17030-050150NmVALID 50/200, NW Rose Plasmid50206-60043-402400
NmPILOT 70/100,NanoGel dT2070105-17030-050150NmPILOT 70/100,NW Rose Plasmid70105-60043-402400
NmPILOT 70/200,NanoGel dT2070205-17030-050150NmPILOT 70/200,NW Rose Plasmid70205-60043-402400
NmPILOT 100/100,NanoGel dT2010105-17030-050150NmPILOT 100/100,NW Rose Plasmid10105-60043-402400
NmPILOT 100/200,NanoGel dT2010205-17030-050150NmPILOT 100/200,NW Rose Plasmid10205-60043-402400
NmPILOT 140/100,NanoGel dT2014105-17030-050150NmPILOT 140/100,NW Rose Plasmid14105-60043-402400
NmPILOT 140/200,NanoGel dT2014205-17030-050150NmPILOT 140/200,NW Rose Plasmid14205-60043-402400
NmPILOT 200/100,NanoGel dT2020105-17030-050150NmPILOT 200/100,NW Rose Plasmid20105-60043-402400
NmPILOT 200/200,NanoGel dT2020205-17030-050150NmPILOT 200/200,NW Rose Plasmid20205-60043-402400
NmTRAP 1mL, NW Rose Plus Plasmid77221-60043-402401NmVALID 35/100, NW Rose Plus Plasmid35101-60043-402401
NmTRAP 5mL, NW Rose Plus Plasmid16255-60043-402401NmVALID 35/200, NW Rose Plus Plasmid35201-60043-402401
NmSCREEN 4.7mL, NW Rose Plus Plasmid77105-60043-402401NmVALID 50/100, NW Rose Plus Plasmid50106-60043-402401
NmPREP 16/100, NW Rose Plus Plasmid16103-60043-402401NmVALID 50/200, NW Rose Plus Plasmid50206-60043-402401
NmPREP 26/100, NW Rose Plus Plasmid26153-60043-402401NmPILOT 70/100,NW Rose Plus Plasmid70105-60043-402401
NmVALID 8/100, NW Rose Plus Plasmid08101-60043-402401NmPILOT 70/200,NW Rose Plus Plasmid70205-60043-402401
NmVALID 8/200, NW Rose Plus Plasmid08201-60043-402401NmPILOT 100/100,NW Rose Plus Plasmid10105-60043-402401
NmVALID 11/100, NW Rose Plus Plasmid11101-60043-402401NmPILOT 100/200,NW Rose Plus Plasmid10205-60043-402401
NmVALID 11/200, NW Rose Plus Plasmid11201-60043-402401NmPILOT 140/100,NW Rose Plus Plasmid14105-60043-402401
NmVALID 16/100, NW Rose Plus Plasmid16106-60043-402401NmPILOT 140/200,NW Rose Plus Plasmid14205-60043-402401
NmVALID 16/200, NW Rose Plus Plasmid16206-60043-402401NmPILOT 200/100,NW Rose Plus Plasmid20105-60043-402401
NmVALID 26/100, NW Rose Plus Plasmid26106-60043-402401NmPILOT 200/200,NW Rose Plus Plasmid20205-60043-402401
NmVALID 26/200, NW Rose Plus Plasmid26206-60043-402401