UniHR Phenyl HIC Pre-packed Column

Hydrophobic interaction chromatography (HIC) separates biomacromolecules based on their differences in hydrophobicity. Under high-salt conditions, biomacromolecules adsorb onto the hydrophobic ligands of the HIC media. By decreasing the salt concentration, proteins are eluted in order of increasing hydrophobicity, achieving separation and purification. HIC offers mild operating conditions and high resolution, making it one of the most commonly used methods for biomacromolecule purification.